Ivanovsky Virological Research Institute of the Russian Academy of Medical Sciences.
Academician S.M. Klimenko of the Russian Academy of Medical Sciences, N.N. Nosik Ph.D. (Medical Science), D.N. Nosik Ph.D. (Medical Science).
а) We conducted a study of the effect of the pyramid field on human lymphoblastic cells. The source of the pyramid field was water that had been exposed in the pyramid and then used to produce a nutrient solution.
The viability of the cells was determined by staining with 0.4% tripan blue (Serva, Germany) and MTT (Sigma, USA) with spectrophotometry of the absorption of the vital dye.
As early as the 10th day of the experiment there began a noticeable (several fold) growth in the number of cells and the percentage of viable cells in the treated sample compared to the control.
Data was obtained on the stimulating effect of a nutrient medium prepared with water exposed in a pyramid on the viability and proliferation of human cells.
An increase in the duration of viability over the control was found. For example, on day 11 these figures were 1.2 million/ml and 52% respectively for the control and 1.4 million/ml and 88% for the experiment; on day 21 0.05 million/ml and 2% for the control, and 0.3 million/ml and 49% for the experiment.
b) In the same place we conducted a study of the effect of the pyramid field on the antiviral activity of immunoglobulin. The subject of the study was venoglobulin — human polyvalent immunoglobulin for intravenous injection. The study was carried out on a culture of human diploid fibroblast cells. To determine the antiviral activity of the immunoglobulin the virus causing encephalomyocarditis (EMC) in mice was used. The antiviral activity of the preparation was determined by its capacity to protect the human cells from the cytopathic action of the virus.
The venoglobulin was dissolved in distilled water in accordance with the instructions to a concentration of 50 μg/ml. In the study the preparation was tested at two concentrations: 50 μg/ml and 0.5 μg/ml. Aliquot quantities of venoglobulin in both concentrations was exposed in the pyramid. The venoglobulin was introduced into cell cultures, 24 hours before they were infected with a virus. The EMC virus reproduces well in diploid cultures of human fibroblasts, producing a pronounced cytopathic effect. The infectious titre of the virus reached 5.0 lg CPD50. Venoglobulin at a concentration of 50 μg/ml significantly inhibited the reproduction of the virus and its titre reached only 2.0 lg TCPD50 (a inhibition factor of 3.0 lg). With the concentration of the preparation reduced 100-fold, a protective effect could no longer be detected.
When venoglobulin preparations of the same concentrations that had, however, been exposed in the pyramid were used a different picture was observed. In this case the preparation at a concentration of 50 μg/ml inhibited the reproduction of the EMC virus by 4.0 lg. Most significant, however, was that the preparation at a concentration of 0.5 μg/ml had the same inhibiting effect. Thus, venoglobulin at a concentration of 0.5 μg/ml that had had no protective effect on the cells, after spending time in the pyramid possessed a more pronounced virus-inhibiting effect than a preparation 100 times more concentrated.
Under further dilution, to concentrations of 0.005 μg/ml and 0.00005 μg/ml with subsequent exposure in the pyramid, the venoglobulin displayed a pronounced anti-viral effect — the titre of the EMC virus reached only 1.0 lg TCPD50.
The anti-viral activity of the venoglobulin practically ceased to depend on its concentration.